Microfluidic electrophoretic mobility shift assays for quantitative biochemical analysis
نویسندگان
چکیده
منابع مشابه
High-Throughput Electrophoretic Mobility Shift Assays for Quantitative Analysis of Molecular Binding Reactions
We describe a platform for high-throughput electrophoretic mobility shift assays (EMSAs) for identification and characterization of molecular binding reactions. A photopatterned free-standing polyacrylamide gel array comprised of 8 mm-scale polyacrylamide gel strips acts as a chassis for 96 concurrent EMSAs. The high-throughput EMSAs was employed to assess binding of the Vc2 cyclic-di-GMP ribos...
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thaliana plants. C. R. Acad. Sci. (Paris) 316:1194-1199. 2.Devic, M., S. Albert, M. Delseny, and T.J. Roscoe. 1997. Efficient PCR walking on plant genomic DNA. Plant Physiol. Biochem. 35:331-339. 3.Doyle, J.J. and J. L Doyle. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15. 4.Frey, M., C. Stettner, and A. Gierl. 1998. General method for gene isolation in tagging approaches: Ampli...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2014
ISSN: 0173-0835
DOI: 10.1002/elps.201300500